目的 建立高效毛细管电泳法(HPCE)同时测定不同批次板蓝根药材中表告依春、尿嘧啶、胸腺嘧啶、腺苷、次黄嘌呤、鸟苷、尿苷7种核苷类成分含量的方法。方法 采用未涂渍标准熔融石英毛细管(75 μm×64.5 cm,有效长度56 cm)为分离通道,60 mmol·L-1硼砂-15 mmol·L-1 β环糊精(pH 9.6)为运行缓冲液,分离电压为20 kV,检测波长为254 nm,毛细管温度为25 ℃,压力进样为5 kPa×10 s。结果 7种核苷类成分的响应峰面积与其相应质量浓度的线性关系良好(r＞0.999 4);加样回收率为97.15%～100.40%。实验表明,不同批次板蓝根药材中7种核苷类成分的含量有所差异。结论 该方法简便、可靠,重复性较好,可用于板蓝根质量的评价和控制。
Abstract
OBJECTIVE To establish a high performance capillary electrophoresis(HPCE)method for simultaneous determination of seven nucleosides, including epigoitrin, uracil, thymine, adenosine, hypoxanthine, guanosine and uridine in Isatidis Radix. METHODS The chromatographic separation was performed on an uncoated fused silica capillary(75 μm×64.5 cm, 56 cm of effective length)with separation voltage of 20 kV. 60 mmol·L-1 Na2B4O7-15 mmol·L-1 β-CD was selected as the running buffer solution(pH 9.6). The detection wavelength was set at 254 nm. The sample was injected at 5 kPa×5 s and column temperature was maintained at 25 ℃. RESULTS The calibration curves of the seven nucleosides showed good linearity (r＞0.999 4). The average recoveries of the method were between 97.15%-100.40%. The contents of the seven nucleosides in Isatidis Radix samples of different batches were different. CONCLUSION The established method is simple, accurate and can be used for the quality control of Isatidis Radix.
关键词
高效毛细管电泳 /
板蓝根 /
核苷 /
同时测定
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Key words
HPCE /
Isatidis Radix /
nucleoside /
simultaneous determination
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中图分类号：
R917
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参考文献
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